FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_H1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_H1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22006399
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC664330.30188037579433147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA456340.20736695722003404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT397880.18080195673994642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281010.12769467644388344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2278100.097.0131151
AGCAGTG2550200.086.530732
GCAGTGG2978600.074.01013
CAGTGGT3182650.069.214274
AGTGGTA3234500.068.172665
TGGTATC3324350.066.201797
GTGGTAT3350000.065.7354666
GGTATCA3359450.065.602158
GTATCAA3431150.064.268379
CAACGCA3435300.036.3406312-13
ATCAACG3450650.036.1887510-11
AGAGTAC3461700.035.79540318-19
GCAGAGT3468150.035.77995316-17
ACGCAGA3518350.035.4396714-15
TACATGG3483450.034.8417722-23
AGTACAT3629550.033.9965620-21
CATGGGG1016700.033.42225624-25
TCAACGC3412750.032.3096712-13
CATGGGA2306100.032.11057724-25
AACGCAG3490850.031.61858714-15
Produced by FastQC (version 0.11.5)