FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_H1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_H1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21291973
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC637570.29944148435656953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA446410.20966117137195317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT382880.17982363588381406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2199100.097.034431
AGCAGTG2460000.086.610482
GCAGTGG2880500.073.85393
CAGTGGT3073400.069.1715244
AGTGGTA3128550.067.984325
TGGTATC3215250.066.057787
GTGGTAT3239650.065.5923546
GGTATCA3247250.065.494168
GTATCAA3315950.064.199379
CAACGCA3324900.036.22548312-13
ATCAACG3334600.036.13981610-11
GCAGAGT3348300.035.74135616-17
AGAGTAC3347600.035.69577818-19
ACGCAGA3400100.035.39555714-15
TACATGG3369500.034.73088522-23
AGTACAT3513650.033.87456520-21
CATGGGG980950.033.72738324-25
TCAACGC3301200.032.26008212-13
CATGGGA2245050.031.80024324-25
AACGCAG3375050.031.53446214-15
Produced by FastQC (version 0.11.5)