FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_G1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_G1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21509474
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC644710.2997330385670984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA446240.2074620699697259No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT389820.18123176791770917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275280.12798081440764195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2209900.097.324761
AGCAGTG2469700.086.970692
GCAGTGG2903150.073.896963
CAGTGGT3091650.069.3480764
AGTGGTA3139750.068.310145
TGGTATC3233450.066.25987
GTGGTAT3253750.065.863536
GGTATCA3267900.065.6536258
GTATCAA3343400.064.2274869
CAACGCA3341650.036.3383812-13
ATCAACG3355250.036.1576510-11
GCAGAGT3375800.035.78146716-17
AGAGTAC3374650.035.68932318-19
ACGCAGA3425750.035.4229514-15
TACATGG3399550.034.6662122-23
AGTACAT3537750.033.88888520-21
CATGGGG984800.033.1179424-25
TCAACGC3317500.032.4072912-13
CATGGGA2260600.031.9670624-25
AACGCAG3400150.031.64579214-15
Produced by FastQC (version 0.11.5)