FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_G1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_G1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21021970
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC622410.2960759624335873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA428450.2038105848310125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT375230.1784942134348018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285670.1358911652904081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2151200.097.106361
AGCAGTG2403500.086.780612
GCAGTGG2815400.073.942283
CAGTGGT2997750.069.353014
AGTGGTA3048900.068.2523655
TGGTATC3135400.066.319827
GTGGTAT3159000.065.83626
GGTATCA3169350.065.7070548
GTATCAA3238550.064.420739
CAACGCA3239050.036.4219812-13
ATCAACG3256500.036.23937610-11
GCAGAGT3273200.035.81725716-17
AGAGTAC3276750.035.74563618-19
ACGCAGA3324650.035.45376614-15
TACATGG3293900.034.78495822-23
AGTACAT3440700.033.8828820-21
CATGGGG958050.033.31668524-25
TCAACGC3219250.032.4225512-13
CATGGGA2186100.032.1610124-25
AACGCAG3290350.031.72277314-15
Produced by FastQC (version 0.11.5)