FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20470548
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC607610.29682156041938884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA422950.2066139118503325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT372690.1820615647416962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2072650.097.316981
AGCAGTG2321600.086.716022
GCAGTGG2730650.073.59623
CAGTGGT2910300.069.0200044
AGTGGTA2962750.067.8643955
TGGTATC3048700.065.853517
GTGGTAT3067600.065.479826
GGTATCA3080250.065.236818
GTATCAA3150150.063.8940129
CAACGCA3155800.036.13351412-13
ATCAACG3167400.035.9945410-11
GCAGAGT3178800.035.63718416-17
AGAGTAC3181450.035.56207718-19
ACGCAGA3222550.035.34662214-15
TACATGG3209750.034.51410322-23
AGTACAT3346850.033.68602820-21
CATGGGG934550.032.98093424-25
TCAACGC3132150.032.16094612-13
CATGGGA2125800.031.75874324-25
AACGCAG3205450.031.4250514-15
Produced by FastQC (version 0.11.5)