FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_G1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_G1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19858766
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC596340.30029056186069164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA416770.20986701792044885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT361900.1822369023332064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2055500.097.210731
AGCAGTG2302600.086.6742552
GCAGTGG2690700.074.068173
CAGTGGT2865000.069.499284
AGTGGTA2917450.068.2858055
TGGTATC2999750.066.3154457
GTGGTAT3018850.065.9482046
GGTATCA3033550.065.655498
GTATCAA3096300.064.354979
CAACGCA3098500.036.4364112-13
ATCAACG3108250.036.30713310-11
GCAGAGT3127750.035.87552616-17
AGAGTAC3126000.035.8407418-19
ACGCAGA3178600.035.50596614-15
TACATGG3149050.034.81020422-23
AGTACAT3280500.034.01873420-21
CATGGGG916150.033.38289624-25
TCAACGC3075450.032.42738712-13
CATGGGA2083400.032.18857224-25
AACGCAG3149750.031.7005314-15
Produced by FastQC (version 0.11.5)