FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23179189
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC693750.2992986510442622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA474050.20451535211175853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT417910.18029535028166863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2370950.097.212051
AGCAGTG2649950.086.8149262
GCAGTGG3123000.073.596153
CAGTGGT3324900.069.102934
AGTGGTA3384950.067.943285
TGGTATC3482350.065.9682167
GTGGTAT3506750.065.552066
GGTATCA3524400.065.2843558
GTATCAA3601550.063.956359
CAACGCA3607650.036.08246612-13
ATCAACG3623700.035.92395410-11
GCAGAGT3642750.035.550616-17
AGAGTAC3642500.035.50385718-19
ACGCAGA3696400.035.19505314-15
TACATGG3665400.034.57394422-23
AGTACAT3824900.033.67166520-21
CATGGGG1071150.033.5770424-25
TCAACGC3580500.032.19571712-13
CATGGGA2433650.031.6758524-25
AACGCAG3671200.031.41455814-15
Produced by FastQC (version 0.11.5)