FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23321986
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC693450.29733745659567756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA485040.2079754271355793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT424210.18189274275355452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317870.1362962828294297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2389950.097.28281
AGCAGTG2669400.086.915172
GCAGTGG3127900.074.059223
CAGTGGT3337550.069.3497244
AGTGGTA3398700.068.1702355
TGGTATC3492300.066.238597
GTGGTAT3522450.065.719916
GGTATCA3538950.065.47398
GTATCAA3617000.064.154789
CAACGCA3623950.036.19598412-13
ATCAACG3638950.036.07335310-11
GCAGAGT3656150.035.64367316-17
AGAGTAC3657750.035.58060518-19
ACGCAGA3708200.035.33008214-15
TACATGG3684850.034.6411522-23
AGTACAT3830300.033.8886720-21
CATGGGG1070100.033.69995524-25
TCAACGC3595800.032.27664612-13
CATGGGA2443900.031.68668424-25
AACGCAG3685100.031.47688714-15
Produced by FastQC (version 0.11.5)