FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22258083
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC662780.29777047735871953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA459470.2064283792993314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT404230.1816104288945279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284990.1280388791793076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2289050.097.367681
AGCAGTG2554300.087.133662
GCAGTGG3000250.074.08973
CAGTGGT3201350.069.377974
AGTGGTA3254550.068.300885
TGGTATC3350400.066.2517857
GTGGTAT3371400.065.877526
GGTATCA3381500.065.704228
GTATCAA3453150.064.390899
CAACGCA3461300.036.23621712-13
ATCAACG3479400.036.06110-11
GCAGAGT3494450.035.68821316-17
AGAGTAC3490950.035.64975718-19
ACGCAGA3544800.035.37886414-15
TACATGG3511300.034.67386222-23
AGTACAT3665200.033.83979420-21
CATGGGG1025250.033.23612224-25
TCAACGC3434900.032.3977312-13
CATGGGA2318300.031.91572424-25
AACGCAG3517050.031.66085214-15
Produced by FastQC (version 0.11.5)