FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_F1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_F1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23314281
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC691400.2965564325144747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA476110.20421388933246537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT425790.1826305516348542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2414850.097.209481
AGCAGTG2690700.087.081632
GCAGTGG3156500.074.1714553
CAGTGGT3360750.069.6157154
AGTGGTA3423100.068.427075
TGGTATC3521000.066.434847
GTGGTAT3545550.066.019866
GGTATCA3553400.065.937648
GTATCAA3631350.064.609729
CAACGCA3624300.036.46147512-13
ATCAACG3645800.036.27149210-11
GCAGAGT3663000.035.8805516-17
AGAGTAC3662600.035.84478818-19
ACGCAGA3713600.035.5682114-15
TACATGG3684850.034.86437622-23
AGTACAT3838550.034.0785620-21
CATGGGG1061250.033.5547924-25
TCAACGC3603250.032.5295412-13
CATGGGA2446000.032.13897724-25
AACGCAG3687350.031.78234114-15
Produced by FastQC (version 0.11.5)