FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22682332
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC673710.29701972442692404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA461510.20346673349107136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT412760.1819742343952994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298390.1315517293371775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2337600.097.019141
AGCAGTG2612150.086.691032
GCAGTGG3058550.073.970653
CAGTGGT3257250.069.389324
AGTGGTA3319600.068.158615
TGGTATC3406650.066.315487
GTGGTAT3431850.065.8976
GGTATCA3442300.065.6834648
GTATCAA3524950.064.1990059
CAACGCA3525700.036.3690912-13
ATCAACG3544200.036.19664410-11
GCAGAGT3556300.035.85179516-17
AGAGTAC3560300.035.78013218-19
ACGCAGA3612450.035.50104514-15
TACATGG3578700.034.87159722-23
AGTACAT3729900.034.02920-21
CATGGGG1044700.033.70808424-25
TCAACGC3502600.032.356212-13
CATGGGA2369600.032.1229524-25
AACGCAG3588450.031.60201514-15
Produced by FastQC (version 0.11.5)