FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_E1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_E1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25538526
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC760250.2976875016201013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA526250.2060612268695539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT463410.18145526488098804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2637500.097.0956041
AGCAGTG2947150.086.703842
GCAGTGG3440850.074.174223
CAGTGGT3671100.069.474894
AGTGGTA3732900.068.3797765
TGGTATC3832850.066.484737
GTGGTAT3868200.065.942896
GGTATCA3878800.065.793128
GTATCAA3972150.064.339989
CAACGCA3967350.036.44804812-13
ATCAACG3989000.036.24936710-11
GCAGAGT4002200.035.89871216-17
AGAGTAC4004300.035.83529318-19
ACGCAGA4064800.035.56085214-15
TACATGG4035650.034.80726622-23
AGTACAT4207200.033.96328420-21
CATGGGG1170500.033.57483724-25
TCAACGC3942500.032.43048512-13
CATGGGA2682800.032.09712224-25
AACGCAG4035550.031.6899214-15
Produced by FastQC (version 0.11.5)