FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20259058
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC602670.29748174865781024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA420970.2077934719373428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT369880.1825751226932664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2079200.097.44291
AGCAGTG2333950.086.6893842
GCAGTGG2735600.073.910633
CAGTGGT2910600.069.482484
AGTGGTA2969350.068.173475
TGGTATC3050850.066.291437
GTGGTAT3075100.065.788796
GGTATCA3082850.065.7040948
GTATCAA3153350.064.314299
CAACGCA3154250.036.28117412-13
ATCAACG3168200.036.10613310-11
GCAGAGT3181250.035.7439316-17
AGAGTAC3176700.035.72072618-19
ACGCAGA3226150.035.42862714-15
TACATGG3199200.034.6749722-23
AGTACAT3339350.033.84362420-21
CATGGGG939450.033.2586324-25
TCAACGC3133100.032.36998412-13
CATGGGA2111850.032.0252624-25
AACGCAG3206150.031.6150314-15
Produced by FastQC (version 0.11.5)