FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n02_dpErk_WE_514hpf_E1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n02_dpErk_WE_514hpf_E1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19807172
Sequences flagged as poor quality0
Sequence length110
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC591530.29864434963254727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGACCTGAGGTCGGACGA405310.20462789942956014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT358680.18108592180650523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG249350.1258887437338354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2035150.097.0610351
AGCAGTG2267550.086.987782
GCAGTGG2662050.074.0488743
CAGTGGT2837750.069.432274
AGTGGTA2883000.068.385355
TGGTATC2966450.066.392497
GTGGTAT2986450.065.956236
GGTATCA2995500.065.805858
GTATCAA3066550.064.359659
CAACGCA3065550.036.33388512-13
ATCAACG3076000.036.22228210-11
GCAGAGT3090850.035.81598316-17
AGAGTAC3092450.035.73280318-19
ACGCAGA3138050.035.47760414-15
TACATGG3105750.034.77330822-23
AGTACAT3240400.033.95341520-21
CATGGGG902100.033.61727524-25
TCAACGC3044000.032.39882712-13
CATGGGA2061900.031.9994424-25
AACGCAG3116350.031.65307814-15
Produced by FastQC (version 0.11.5)