FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51802952
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG752590.1452793655465812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA28350.016.9050921
GCCCGAA20100.016.0602721
CTGGACG20450.015.6776521
GCGGGGG18000.015.5861932
GGGCTAC23150.015.5148631
GAGACCC26900.015.2769941
CGGGGGG18750.015.2541293
GGCGGGG18450.015.2273781
GGGACCT22350.015.1335981
ATGGGAG21750.015.0951821
CTGGCAG28700.014.664351
AACGGGA17900.014.5873581
TGCGGAG18250.014.3459553
AGGGAAG14050.014.3281523
TGCGACG24050.014.1096751
CCGGGTA24050.013.9722431
GGGCCAT25700.013.9325821
GGGATCC26650.013.89067651
AGCCACG29950.013.7948121
CGGAGAA23400.013.6541131
Produced by FastQC (version 0.11.5)