FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_H1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_H1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18991133
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA39850.016.978381
GCCCGAA27600.015.82453251
CTGGACG28400.015.688671
ATGGGAG28400.015.378771
GGGCTAC32050.015.3093341
GAAGCCC40100.015.0069491
TGCGACG31800.014.9799451
GAGACCC39950.014.9531431
AAACGAA34300.014.9144861
GGGACCT27000.014.8723141
TGGAACT35650.014.7817361
TTGACCC36050.014.6787561
AGCCACG37900.014.6589081
AATGGAA42650.014.6513881
CTGGCAG35450.014.4927261
TCGGGAC27800.014.4839081
GGCACGT32600.014.4436051
GCGGAAA25000.014.3898811
AAACCCA35300.014.3673161
AGGCTGC34100.014.3567161
Produced by FastQC (version 0.11.5)