FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_H1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_H1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21291973
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA44100.017.2389241
GCCCGAA28850.016.3599571
CTGGACG30450.015.7171071
GGGCTAC34150.015.5606271
AAACGCT39550.014.9938431
TGCGACG37650.014.8446311
GAAGCCC46450.014.803521
GAGACCC45950.014.7012261
AAACGAA36200.014.649041
GGGACCT30500.014.6452531
TTGACCC40450.014.6058361
GGGCCAT38800.014.6031381
ATACCGA35150.014.5858341
AATGGAA45950.014.5575661
TCGGGAC31100.014.5042121
GGGATCC39650.014.4565681
AGCCACG40750.014.4443111
TGCGGAG25450.014.3926393
TCGAGAA37300.014.3905928
AGGGAAG23850.014.3896483
Produced by FastQC (version 0.11.5)