FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_G1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_G1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20470548
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA41050.017.018621
GGGCTAC33650.015.8896731
CTGGACG30850.015.7270481
GCCCGAA29300.015.6578531
GGGACCT30100.015.351351
GAGACCC41000.015.1610171
AGGGAAG22650.015.1519843
TGCGACG35150.015.1489951
TGCGGAG28550.015.025943
GAAGCCC47050.014.9184891
AAACGCT39650.014.8170531
AGCCACG42750.014.7720081
ATGGGAG31650.014.6690681
AAACCCA38050.014.5437741
GCGACGA14550.014.4396512
CCCGAAG34100.014.3571881
TTGACCC39100.014.3501761
AAACGAA35600.014.3394251
GGGATCC37700.014.2702411
TGGAACT37500.014.2583331
Produced by FastQC (version 0.11.5)