FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_F1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_F1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23179189
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA46550.017.3951471
GAAGCCC49050.015.7234921
GCCCGAA32800.015.5972891
CTGGACG33800.015.4613311
GAGACCC52300.015.4195711
GGGCTAC37200.015.1129051
GAACGAG28550.014.9875323
TGCGACG41400.014.8818761
AATGGAA50950.014.8564211
AAACGCT42100.014.7912311
AAACGAA43500.014.7704461
GGGATCC40950.014.6155441
CCCTGAT40100.014.5412431
AAACCCA40800.014.4805191
TGGAACT46050.014.3347991
TATATCC45200.014.3122821
AGCCACG47900.014.3094361
AACGGGA29050.014.2779281
GGGCCAT42000.014.2501551
GGGACCT32700.014.2318911
Produced by FastQC (version 0.11.5)