FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_F1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_F1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23321986
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA31300.016.9068031
CCGGACA48550.016.7688481
GGGCTAC36250.015.6907361
GAGACCC49050.015.5437211
CTGGACG35950.015.148411
TGCGACG38300.014.8796171
GAAAAGA16950.014.8612654
TGGAACT46350.014.7401741
GGGACCT33450.014.7347461
ATGGGAG36650.014.7089911
GCGGAAA31050.014.63353351
GGGATCC43850.014.6020591
AAACGCT43000.014.5325111
CATGACG33500.014.5126588
AGCCACG47700.014.4844511
AATGGAA51300.014.4545071
GCGACGA16050.014.3925272
TGGGCTG43650.014.3413091
AGGCTGC44100.014.2698111
TGCGGAG30250.014.25445653
Produced by FastQC (version 0.11.5)