FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_F1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_F1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22258083
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA45150.017.3984321
GGGCTAC35350.015.6548231
GCCCGAA30400.015.4895871
CTGGACG32800.015.3624781
GGGATCC40900.015.3327891
GGGACCT31800.015.2574211
TGCGACG42800.015.0634171
GAGACCC45750.015.0059371
AGTAAAC38350.014.8861318
ATGGGAG36450.014.8201841
GAACGAG28850.014.7175323
AAACGAA39200.014.4821581
AAACGCT40300.014.4690661
GAAGCCC47500.014.4530871
AATGGAA50350.014.3560691
AAACCCA45100.014.2952161
AGCGCCA49050.014.2879541
TGGAACT40750.014.2822851
CTGGCAG43500.014.2393051
TGCGGAG30100.014.1428843
Produced by FastQC (version 0.11.5)