FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_F1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_F1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23314281
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA44300.017.3348481
CTGGACG36250.015.8124071
GGGCTAC35950.015.5771211
GCCCGAA35000.015.2140781
GAGACCC50250.015.1508971
TGCGACG39400.015.0508431
AAACGCT43700.014.9041971
GAAGCCC53550.014.8335811
TGGAACT43250.014.8303281
AATGGAA53800.014.7851031
AAACGAA40900.014.74095251
CGAAAAG25150.014.6081353
AACCTGA38050.014.5439261
AGCCACG49050.014.489781
GGGACCT34500.014.4778831
GAACGAG28150.014.4189723
AAACCCA42300.014.357111
GGGATCC41950.014.3195381
TCGAGAA43150.014.2496238
GAAAAGA17700.014.23144
Produced by FastQC (version 0.11.5)