FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_E1_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_E1_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22682332
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA44300.016.8626061
GCCCGAA31800.016.3295251
CTGGACG34050.016.122861
GGGCTAC36850.015.1963391
TGCGACG40650.015.0748611
GAAGCCC47400.015.017051
TTGACCC39900.014.7516141
AATGGAA49800.014.735171
GGGACCT31750.014.7266451
ATGGGAG33900.014.7013471
GGCACGT39900.014.6688941
AAACGAA41650.014.66008951
AAACGCT40350.014.641631
AGCCACG45850.014.5649171
GAGACCC44750.014.5541661
AGGCTGC40550.014.460891
GAACGAG27050.014.4360043
TGCGGAG31450.014.409953
AACGGGA26450.014.3084121
TCGAGAA38850.014.2695748
Produced by FastQC (version 0.11.5)