FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_E1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_E1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25538526
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA50650.017.2242551
CTGGACG36050.015.9603771
GCCCGAA37400.015.8549141
AAACGCT45500.015.4744531
GAGACCC50400.015.4543041
GAAGCCC56300.015.2416831
ATGGGAG42350.015.2226731
GGGCTAC40850.014.919851
GGGATCC47650.014.8916741
GGGACCT38000.014.8228971
AAACGAA44700.014.8161621
AATGGAA55950.014.6881551
TTGACCC49300.014.6387391
TGCGACG44150.014.6269631
AACGGGA31100.014.574161
AGCCACG52900.014.536781
TGGGCTG44550.014.322771
GCGGAAA33400.014.2293161
AACCTGA40900.014.2291541
TCGAGAA45000.014.2260698
Produced by FastQC (version 0.11.5)