FastQCFastQC Report
Thu 12 Sep 2019
H7JYGBGXC_n01_dpErk_WE_514hpf_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYGBGXC_n01_dpErk_WE_514hpf_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20259058
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA38400.016.5884231
CTGGACG28850.015.7493271
GCCCGAA29200.015.2591351
GGGACCT28300.015.0835311
AAACCCA36600.014.9995141
GAAGCCC45650.014.9660981
AAACGCT36100.014.9329841
ATGGGAG30900.014.8112791
GAGACCC39450.014.7804141
GGGCTAC33300.014.7679781
TGCGGAG26300.014.7642113
AAACGAA32850.014.6353731
AGGGAAG20850.014.6138613
GTGCTGG32600.014.5113751
TGCGACG34150.014.464831
TCGGGAC27600.014.4296971
AATGGAA44450.014.4296081
AGTAAAC31750.014.37748058
GGGATCC38700.014.3561551
AGGCTGC37700.014.2992241
Produced by FastQC (version 0.11.5)