FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72746928
Sequences flagged as poor quality0
Sequence length55
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28341503.8959033431624768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA7500.016.33641649
GTATCAA25600.015.5907511
CGGTTCG7150.011.65123412
ATCAACG35400.010.9280612
GTATAGA14850.010.5529271
TCTCGGT8400.010.50188748
GTATTAG10000.010.2841561
GTACCGT6200.010.27028756
TACCCCG3751.6996819E-79.7957495
TTCGTTA6300.09.72405749
TAGGGCT11550.09.328474
GTATATA19600.08.9948891
CGCAGAG48650.08.9610587
AGCGGTT14250.08.939404510
GAGTACG12150.08.867391
TAGACTG10250.08.8400665
GATCTCG11100.08.83054946
GTGTATA13700.08.7577971
CTAGGGT8750.08.6754764
TATACTG12600.08.5518445
Produced by FastQC (version 0.11.5)