FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n02_DS_CCF_2_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n02_DS_CCF_2_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71621031
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA382800.021.9780311
GGTATCA194850.018.3719181
TCAACGC440850.017.6248684
CAACGCA470150.016.4811555
TATCAAC512600.015.6815812
TCTCGGT124850.014.70084148
AACGCAG536750.014.5236336
ATCAACG566750.013.8296183
GTATAGA145000.012.5128941
ACGCAGA644800.012.0260087
CTCGGTG157600.011.84819549
GTATTAG126650.011.6772161
GATCTCG164600.011.19566146
ACGTCGT39150.011.01487223
GTACGTA65350.010.9406931
TATACTG169750.010.7625875
GTATAAT220700.010.7505081
CGCAGAG724700.010.7211518
CGCGAAT33950.010.68062128
ATCTCGG173050.010.62032147
Produced by FastQC (version 0.11.5)