FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n02_DS_CCF_2_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n02_DS_CCF_2_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62537630
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168250.020.8398511
GTATCAA340000.020.690141
TCAACGC389150.016.864164
TCTCGGT103700.016.42321448
CAACGCA423550.015.4609195
TATCAAC464850.014.5586042
AACGCAG483200.013.6131225
CGTCGTT37550.013.37854746
ATCAACG506900.013.0470963
CTCGGTG134150.012.89650649
GTATAGA129150.012.8730691
GTATTAG109700.012.6332911
CGTCGTC34950.012.26774724
ACGTCGT36500.012.08305723
GATCTCG143400.011.94518946
GTATAAT217550.011.8290631
CCTATAC167950.011.7361973
ATCGTCG44900.011.51578344
CGCGAAT41100.011.32638428
GGGGTAA166500.011.3184236
Produced by FastQC (version 0.11.5)