FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n02_DS_CCF_2_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n02_DS_CCF_2_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72262849
Sequences flagged as poor quality0
Sequence length55
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA389700.021.2240681
GGTATCA189950.019.7993661
TCAACGC456350.016.8959663
CAACGCA492700.015.7101424
TCTCGGT112200.014.69869948
TATCAAC543600.014.6879392
AACGCAG559300.013.9850425
CGCGAAT66250.013.23960428
ATCAACG596350.013.2080682
GTTCGCG20100.013.04512838
GTATTAG129650.012.9931761
GTATAGA140000.012.5922651
GGGGTAA226950.012.42854236
ACACGGT140350.012.2689758
CGTCGTT41200.012.25292146
GTATAAT241600.012.060061
ACGTCGT40000.011.82209523
CGAGTAT163650.011.78145718
CCGCGAA76550.011.7143627
GGGCGTA79400.011.6928129
Produced by FastQC (version 0.11.5)