FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n02_DS_CCF_2_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n02_DS_CCF_2_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87102576
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA499500.023.2454451
GGTATCA215650.022.0728591
TCAACGC633350.017.482283
CAACGCA683350.016.2831864
TATCAAC739800.015.2310352
ATCAACG767450.014.7141872
AACGCAG777650.014.4327825
TCTCGGT144050.013.79608248
GTCGAAC67350.012.91495823
GTTCGCG33250.012.45532838
GTATTAG124150.012.247761
ACGCAGA945850.011.8488546
GTATAGA156300.011.6866931
GTATAAT271850.011.6371071
CGTCGAA75500.011.58534522
CGTCGTT45850.011.2239646
TATACAG359750.011.1437735
CGCGAAT49450.011.04888228
CGCAGAG1029150.011.0282457
ACGTCGT50000.010.92790923
Produced by FastQC (version 0.11.5)