FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72746928
Sequences flagged as poor quality0
Sequence length20
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT17500.012.2835091
GTTCCCA31750.012.2176641
GTCCGAA19850.012.1344491
GTCCCAT29950.012.1336841
GTGCAAT19600.012.1106031
GTCTCTA21200.011.890191
GTCCTCT34750.011.8882891
GTACTCA16350.011.734241
GTTCTCC24450.011.7129581
GTGTCAC15650.011.6774591
GTCTACG18000.011.6700011
GTGTAAC17050.011.66315651
GTTATCA23900.011.6602361
GTCAGCA30500.011.6393921
GTTTAAC24650.011.6179241
GTACCAA20550.011.5848191
GTAGTAC9450.011.5588591
GTCATTA28700.011.5154871
TCATGTA21500.011.4933052
GTCATAC26750.011.4911351
Produced by FastQC (version 0.11.5)