FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n01_DS_CCF_2_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n01_DS_CCF_2_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71621031
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAC264750.013.2688891
ACTGTTA351550.013.15348154
ACAAAAT229800.013.1315884
CTCGTAC187350.012.998626
GTTGATC273300.012.9972271
GTCATAA379500.012.9959821
CTGTTAA359550.012.987555
GTATCCC374700.012.9849721
GTTCCCA304250.012.9820231
GACACTA163250.012.9577153
GTATGCA252600.012.9565131
GGACCAA143500.012.9460794
GTATAAA237500.012.9401941
GTAACAA163650.012.8976681
CCTATAA208050.012.8896456
GTCAAAA141250.012.8762851
GTTTCCT341750.012.8705891
GACAGCA140100.012.8607425
GTCCCAC187000.012.822091
CGGAACC162550.012.7984515
Produced by FastQC (version 0.11.5)