FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n01_DS_CCF_2_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n01_DS_CCF_2_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62537630
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT152150.012.920161
GTCGCTC241450.012.89965251
GTACTCA123850.012.7861381
ACCAAAT126600.012.7004384
GTAAGTA128550.012.6563021
GTCCGAA145550.012.6546881
TCTAAGA178400.012.6265892
CAGCAGA146900.012.6083233
GTTATCA151100.012.537361
GTCAAAA119950.012.4898241
GTACATA114500.012.4545591
GTTGTTC220950.012.4235471
CTTCGCC233200.012.3908017
GTGGAAC153100.012.3827271
TTACCAA139700.012.3313342
TCCTGAA179950.012.3272232
GTCTCTA171300.012.3176731
GTGCCAC113500.012.2990651
GTGACTC110500.012.2971951
ATACGTC202900.012.2921528
Produced by FastQC (version 0.11.5)