FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n01_DS_CCF_2_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n01_DS_CCF_2_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72262849
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCC200350.012.9762165
GTCGCAA239200.012.9708481
GTCACCA197000.012.8212421
GTACTAA117600.012.8045071
GCAAACT209050.012.7540984
GTACTAT162900.012.737581
GCACGAA110350.012.7310434
GTGATAA155450.012.5959671
GTGTCCA236500.012.5032411
CCCGTAA190700.012.4949548
GTTTTAC251900.012.48364451
GTCCCCT304450.012.4834221
GTCCGCA261450.012.3891271
GTGAAAC139200.012.341421
GTAGAAT179750.012.3224591
ATCGGAC210400.012.2763343
GTGATCA158250.012.2138481
GTCAAAC133250.012.1780011
GTGACCT128750.012.1740941
GTAGCAA123550.012.1651951
Produced by FastQC (version 0.11.5)