FastQCFastQC Report
Tue 24 Sep 2019
H7JYFBGXB_n01_DS_CCF_2_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JYFBGXB_n01_DS_CCF_2_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87102576
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT636600.013.5789721
AGCCATA422850.013.5642787
GTACTCA244650.013.5519981
GCCAATA402950.013.5464134
TTGCCAA403500.013.4950182
TCGATCA529200.013.4785573
TACCAAA405500.013.4664372
CAAGCAA318300.013.45455555
GCCATAC364850.013.4453488
GTACCAA428750.013.44446851
AAGCAAC317400.013.4397996
AAGCCAT374250.013.4351126
GATATCA262600.013.4346043
GTCTCAA416950.013.4186371
TCGAATT503200.013.4086362
GTAATGA413950.013.4008861
CAAAGCC400300.013.3879135
CAAGCCA379850.013.3807615
GCAACGC426900.013.3357338
ATCACAA219000.013.3351366
Produced by FastQC (version 0.11.5)