FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39088557
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1123310.28737566341985965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA407950.056.8950962
GATCGGA412650.056.023291
TCGGAAG424000.053.0246283
CGGAAGA431550.050.880414
ACACGTC379350.045.39753313
CACACGT392100.044.92860812
GCACACG414700.043.0960111
CACGTCT395000.042.6942114
ACGTCTG387650.041.8699615
CGTCTGA387150.041.35436616
AGAGCAC485950.040.4046028
AGCACAC495400.038.4763410
GAAGAGC606850.034.084356
GAGCACA586050.033.0854959
CTGAACT480350.032.35728519
AAGAGCA643500.032.1870467
TGAACTC504950.030.81566620
GAACTCC485650.030.80148721
GGAAGAG736650.029.2846185
AACTCCA560550.027.42877222
Produced by FastQC (version 0.11.5)