FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26243017
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1892100.7209917975513258TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG226250.062.57807543
CTCGTAT227000.061.5107742
TATGCCG233750.060.4907446
CGTATGC234900.060.27357544
TCTCGTA236750.058.68322841
ATGCCGT243750.057.91850347
GTATGCC245250.057.77163745
ACGGCTA247250.057.3645532
ATCTCGT243700.056.9666840
ATCGGAA265900.055.7017862
ACACGTC270650.053.716713
GCTACAT260800.053.63417435
TACATCT260150.053.21377637
CGTCTGA274300.052.77094316
GATCGGA281300.052.6160241
ACGTCTG276900.052.3140615
CTACATC265200.052.0189536
TGCTTGA276050.051.13329358
GCTTGAA277550.050.92009759
CCGTCTT278350.050.88584550
Produced by FastQC (version 0.11.5)