FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27119470
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2144580.7907897905084428TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG249100.063.0511343
CTCGTAT249300.062.34213342
CGTATGC257350.061.46454244
TATGCCG260200.060.78481746
TATCTCG260700.059.91084739
TCTCGTA259700.059.9003641
ATGCCGT268650.058.69738447
GTATGCC272900.058.22968345
ATCTCGT268300.058.1379740
TAGCTTA285400.057.3040134
AGCTTAT285500.057.08828735
GCTTATC278600.057.04443436
ATCGGAA304400.055.5428852
ACACGTC306000.054.73951313
GTCACTA305850.054.13938529
GATCGGA314650.053.7679861
CGTCTGA310300.053.7194616
ACGTCTG313150.053.29839715
CTTATCT296050.053.21612537
ACTAGCT310050.053.1418432
Produced by FastQC (version 0.11.5)