FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28577623
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC9368533.278274753642037TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1098800.065.634782
GATCGGA1104850.065.167221
TCGTATG1083850.064.1820143
CTCGTAT1052250.064.0894542
TATGCCG1087650.063.82102246
CGTATGC1091600.063.7419244
TCTCGTA1059150.063.42153541
ATGCCGT1094600.063.38311847
ACGATCA1102000.063.2527932
TCACGAT1106250.063.02232730
GTATGCC1103200.062.98229645
ATCTCGT1065450.062.89287640
ACACGTC1132400.062.81122213
TCGGAAG1149500.062.5692753
CGTCTGA1134150.062.36915216
CACGATC1121000.062.2179631
GTCACGA1124700.061.96866229
ACGTCTG1143500.061.92432415
CGATCAG1121850.061.8917333
CACACGT1156550.061.4926412
Produced by FastQC (version 0.11.5)