FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28008948
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC17206406.143179672438965TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG387050.13818798192634726No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC380860.13597797389605634TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2188750.064.869932
CTCGTAT1947400.064.84238442
AATCTCG1969700.064.8185139
TCGTATG1990150.064.7174943
TATGCCG2002500.064.6921146
TTGAATC2024950.064.66650436
ATGCCGT1995400.064.6606747
TCTCGTA1958600.064.6062241
GCTTGAA1995900.064.5614159
TGAATCT1997650.064.5296137
TGCTTGA1975300.064.499358
CGTATGC2012000.064.48913644
GTATGCC2023800.064.4523545
ACACGTC2188300.064.3710513
ACGTCTG2180900.064.3682315
GATCGGA2201750.064.363731
ATCTCGT1979700.064.33121540
CGTCTGA2184750.064.2716216
GAATCTC1998500.064.2013738
CTTGAAT2119450.064.0278135
Produced by FastQC (version 0.11.5)