FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26753347
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC974250.3641600432274885TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC120800.059.6010244
TCGTATG120550.059.4639843
CTCGTAT119150.058.842542
TCTCGTA119450.058.5193341
TATGCCG127750.056.518646
ATGCCGT128300.056.1087647
TGCCGTC131850.054.51812448
GATCGGA138000.053.9187281
ACACGTC136900.053.7635713
ATCGGAA140950.052.9878042
CGTCTGA138250.052.85745216
GCCGTCT136150.052.51491549
ATCTCGT134300.052.18024440
CCGTCTT137950.051.8571950
ACGTCTG142900.051.2357115
CATCTCG142600.049.02164539
CGTCTTC146800.048.80390551
CACGTCT151800.048.43936514
TCGGAAG156950.047.652953
CGGAAGA160950.046.49044
Produced by FastQC (version 0.11.5)