FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30260295
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2658200.8784448400122999TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC317450.062.8476944
TCTCGTA303750.062.828841
TCGTATG317300.062.7453443
CTCGTAT306150.062.72469742
ATGCCGT324500.061.45085547
TATGCCG324700.061.3097846
ATCACGA325750.061.0584934
TCACGAT324450.060.753235
ACGATCT312650.060.74769237
GATCTCG313150.060.73115539
CACGATC312800.060.67791736
CGATCTC313700.060.5445638
TGCCGTC332250.060.0806648
ATCTCGT318300.059.81511740
GCCGTCT335950.059.37915849
CCGTCTT336750.059.19955450
CATCACG338850.058.9354633
CGTCTGA348150.058.6660716
ACACGTC351700.058.3240313
ACGTCTG353750.057.797215
Produced by FastQC (version 0.11.5)