FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34860844
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5039221.445524382599572TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG579750.065.2166243
TCTCGTA560250.065.1651541
CTCGTAT561800.065.1217842
CGTATGC581750.064.9677944
AATCTCG567550.064.3335239
ATGCCGT587800.064.092247
TATGCCG588750.064.0721346
ATCTCGT574300.063.59629440
TGCCGTC594900.063.20355648
GCCGTCT594750.063.1625649
CCGTCTT598800.062.7681850
CGTCTGA616100.062.7132316
ACACGTC619100.062.6144613
ACGTCTG622150.062.1882115
CGTCTTC607050.061.79540351
CACGTCT640150.060.5381614
GTATGCC634350.059.66226245
ATCGGAA664950.058.453432
GTAATCT629550.057.9572537
GATCGGA669750.057.899051
Produced by FastQC (version 0.11.5)