FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31284355
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4633971.4812419818148719TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG532300.064.5002243
TCTCGTA520850.064.3217341
CTCGTAT523500.064.2294442
CGTATGC541450.063.95314444
ATGCCGT541650.063.569647
TATGCCG544250.063.46489746
CGTCTGA574700.062.74212316
ATCTCGT536300.062.71231540
TGCCGTC547350.062.68372348
ACACGTC579100.062.55729313
GCCGTCT546850.062.49994749
CCGTCTT550900.062.25885450
CATCTCG542400.061.9610739
ACGTCTG583050.061.89179615
CGTCTTC554800.061.34984651
CACGTCT593350.060.9530714
ATCGGAA604800.060.632592
GATCGGA605550.060.4018331
TCGGAAG615450.059.498083
GTATGCC585200.059.25483745
Produced by FastQC (version 0.11.5)