FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32049135
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1455600.45417762445070675TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG178750.059.82116343
CTCGTAT180900.058.6867142
CGTATGC185150.057.86641344
TATGCCG186750.057.2922746
TCTCGTA188650.056.3135941
TATCTCG189350.055.95752339
ATGCCGT196850.054.38318347
CGCCAAT199950.053.2396933
ATCTCGT200200.053.17046740
GTATGCC208650.051.43222445
CAATATC208200.051.08024636
CCAATAT214350.049.8430135
GCCAATA214550.049.74720834
ACGCCAA224600.047.72248532
ATATCTC223100.047.63279738
CCGTCTT231600.046.2565450
GTCACGC236850.045.33741429
CGTCTGA241000.045.26508316
AGTCACG239800.044.79210328
ACACGTC246450.044.56335413
Produced by FastQC (version 0.11.5)