FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29495980
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC764680.2592488874755136TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG105450.056.06292343
CTCGTAT104550.052.96528642
TATGCCG114650.052.107946
CGTATGC114100.052.05772844
TCTCGTA113800.048.47630341
ATGCCGT124350.048.04001247
ATCTCGT121650.045.2916840
GTATGCC135100.044.30085845
CGTCTGA157000.039.76742616
ACACGTC163000.038.5840213
CCGTCTT157500.037.9327250
ACGTCTG170200.036.78635815
AGTGATC153250.036.47756236
TGCCGTC165050.036.38431548
GATCTCG154100.036.04932839
GTGATCT161950.034.4731737
CACGTCT186500.033.60900514
GCTTGAA182950.032.73223559
CACACGT194300.032.47595212
TGCTTGA185500.032.4333858
Produced by FastQC (version 0.11.5)