FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30879677
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3840771.2437856781986416TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG467050.063.38004343
CTCGTAT447350.062.92581642
CGTATGC476100.062.2117444
TATGCCG475850.062.0705146
TCTCGTA458200.061.19258541
ATGCCGT485050.060.89401647
AATCTCG460800.060.72498339
ATCTCGT463550.060.37395540
GTATGCC494200.059.88220645
ATCGGAA523700.059.308722
ACACGTC518200.059.19712413
GACCAAT500900.058.51600335
GATCGGA531750.058.306881
CGTCTGA524400.058.25489816
ACGTCTG529050.057.7172615
ACCAATC490050.057.0180636
CACGTCT542650.056.4449614
CCGTCTT523700.056.40468250
CACACGT550650.055.8343812
TGCCGTC531750.055.578248
Produced by FastQC (version 0.11.5)