FastQCFastQC Report
Tue 17 Sep 2019
H7JWTBGXC_n01_GG038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JWTBGXC_n01_GG038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34310567
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC503980.1468876920629146TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG75750.050.96753743
CTCGTAT77450.048.9012542
TATGCCG82500.046.4970246
CGTATGC83600.046.18137744
TCTCGTA88050.043.1339841
CGATGTA90500.043.0120334
TATCTCG89100.042.62479839
ATGCCGT93900.040.84766847
ATCTCGT100800.037.748140
ACCGATG105500.037.260232
CCGATGT107450.036.35723533
GTATGCC108250.035.92334745
CACCGAT112300.035.0348331
GATGTAT117750.033.47456735
ATGTATC121950.031.6612536
GTATCTC128100.029.97471838
CCGTCTT135200.028.63018450
TGCCGTC141200.027.56059648
TCACCGA151600.025.95246930
CGTCTGA154700.025.81273816
Produced by FastQC (version 0.11.5)