FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35838728
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32928309.187909794119925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCTA50800.018.60673320-21
TACAGTT68450.013.53168824-25
GGTGCCT76500.013.10063718-19
ATGGTGC82700.011.97452616-17
TCGGAAG178900.011.4929453
ATCGGAA187850.011.4577122
GATCGGA184300.011.4556221
CGGAAGA190300.011.4019964
TGGTGCC91700.010.92910218-19
TCGTGTA96750.010.25977714-15
ATTGATG105500.09.58905512-13
CGTGTAT24450.09.22693314-15
AGCGTCG119700.08.27286610-11
CTCGGGT23350.08.1458071
ACAGTTT89100.08.10367826-27
TTCCCTA36050.08.0361618
CACTCTT63750.07.9001722
GTAGGGA95750.07.78811318-19
CCGATCT24750.07.48686932
CGGGGAT32200.07.2360451
Produced by FastQC (version 0.11.5)