FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16056061
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT33500.010.4961621
TACCGTA14800.010.2699469
GGGGGGA179950.010.108112
CGGGGTT50900.09.6153061
CTACCGT18500.09.499648
CGGCAAT28800.09.2393031
CGGGGAT54250.08.8463741
CGGGAAT38350.08.7970421
GTCGATT12201.4188117E-108.5685341
CGCGGAT22400.08.4850741
GTCGAAT15750.08.4473621
CGCGTAC10304.179674E-88.3038391
CGGATCG11654.7475623E-98.1521163
CCGTAAT15655.456968E-127.89411
CGCGGTT20750.07.78582241
CTCGTAT17750.07.7632451
GGCGAAT22600.07.5689861
GATCGGA226700.07.46296194-95
CCGGGAT46250.07.397151
GCGTCGT95850.07.387658694-95
Produced by FastQC (version 0.11.5)